The Protein and Metabolite Analysis Facility provides services, self-service equipment, and collaborative research for the detection, characterization, and quantification of biomolecules. The facility labs are located in MBB 1.420 (ICMB) and PHR 1.110 (College of Pharmacy). The facility is supported by the Cancer Prevention Research Institute of Texas Shared Instrumentation Award PR110782, Center for Research on Environmental Disease, NIEHS center grant ES007784, and by institutional funds provided by ICMB, UT-Austin, MDACC-Science Park, and the College of Pharmacy.
News and Announcements:
Maria Person is presenting a talk entitled "FT-MS proteomics and other new developments at the Protein and Metabolite Analysis Facility" on Thursday, April 12 at 11:00 a.m. in PHR 4.114 for the Toxicology Seminar (PHR 196T).
The Luminex service will be discontinued effective April 1. Please contact Cissy Geigerman if you are interested in partnering with Dr. Steven Hursting in conducting Luminex analyses with his laboratory.
Marvin Mercado is lab manager for the PHR labs. Please contact him for protein and peptide MW determination services and access to PHR 1.110.
If you are interested in collaborating on a protein modification or quantitative proteomics project or conducting a cancer research proteomics experiment on the state-of-the-art Orbitrap Elite mass spectrometer, contact Maria Person.
Mass spectrometry:
Protein/peptide molecular weight determination by ESI or MALDI
Protein identification by LC-MS/MS and database search for individual gel bands and complex mixtures
Relative quantitation of peptides/proteins with spectral counting or stable isotope labeling
Protein modification analysis for phosphorylation, methylation, acetylation, ubiquitinylation, glycosylation, inhibitor binding, and chemical adduct site determination
Fatty Acid Methyl Ester quantification
Other MBB lab services/instrumentation:
Edman chemistry N-terminal Sequencing
Peptide synthesis
Separation of proteins/peptides by HPLC or FPLC (4°C or room temperature)In-gel and solution proteolytic digest
IMAC and MOAC enrichment of phosphopeptides
Luminex antibody bead based quantitation:
TNF alpha, MCP-1, adiponectin, resistin, insulin, leptin, GM-CSF, Interferon-gamma, IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12(p70), IL-13, IGF1, PAI-1, mTOR, phosho-mTOR, ERK 1/2, phospo-ERK 1/2, AKT/PKB, phospho-AKT/PKB, p70 S6 kinase, phospho-p70 S6 kinase. New assays can be implemented using kits available from vendors.
Self-service instrumentation/ training available:
SDS-PAGE
Peptide synthesis
HPLC and FPLC (4°C or room temperature)
Crystallography robot
Luminometer
MALDI-TOF MS molecular weight determination
When using core services, remember to cite the Protein and Metabolite Analysis Facility in your publications, and funding sources ES007784 and PR110782 when appropriate, and send the citation to Maria Person or inclusion on our website. Continued support for the facility depends on documented outcomes.
Maria D. Person, Ph.D.
Director
Stony H. Lo, Ph.D.,
Manager
Jacqulyn Tolson,
Research Assistant
Mailing Address:
CRED Analytical Facility Core
College of Pharmacy
The University of Texas
at Austin
1 University Station
A1900
Austin, TX, USA
78712-01200
Email Address: mperson
@mail.utexas.edu
Phone:
(512)232-4340
